FB2026_03 , released September 17, 2026
Recombinant Construct: P{Ubi-CasExpress.DQVA}
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General Information
Symbol
P{Ubi-CasExpress.DQVA}
FlyBase ID
FBtp0112156
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Description

Ubi-p63E regulatory sequences drive expression of a fusion protein consisting of Mmus\Cd8a and the GAL4 coding region, separated by sequence derived from Diap1. The Diap1 sequence consists of the caspase-3-binding and cleavage domain (amino acid residues 2-147) in which the DQVD caspase cleavage site has been changed to DQVA (residues residues 21 and 22 have also been mutated from NN to GV). This construct serves as a caspase-insensitive control in the CasExpress system, as the mutated DQVA site can no longer be cleaved by caspase: the Mmus\Cd8a sequence tethers GAL4 to the plasma membrane, rendering it inactive.

(Ding et al., 2016)
Construct components
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
65420
w*; P{Ubi-CasExpress.DQVA}attP40
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
DQVA control
(Ding et al., 2016)
P{Ubi-CasExpress.DQVA}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)