UASp regulatory sequences drive expression of the Cele\unc-104 motor domain (amino acids 1-389) along with a leucine zipper for dimerization, fused to the mutated C-terminus of Khc (amino acids 905-975; with R914A, K915A, R916A and Q918A mutations in the microtubule binding site). The construct is tagged with mTagBFP2 at the C-terminal.