FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{αTub(FRT.y+)FLAG-hpo.4A-431T}
Open Close
General Information
Symbol
P{αTub(FRT.y+)FLAG-hpo.4A-431T}
FlyBase ID
FBtp0126664
Feature type
transgenic_transposable_element
Component Allele(s)
hpo4A-431T.αTub84B.FRT.Tag:FLAG
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele hpo4A-431T.αTub84B.FRT.Tag:FLAG
Transgenic product class
missense_variant
(Zheng et al., 2017)
polypeptide_post_translational_processing_variant
(Zheng et al., 2017)
Component
Use(s)
Regulatory region(s)
αTub84B
Encoded product / tool
hpo
Tagged with
Tag:FLAG
Also carries
FRT
Description

An FRT cassette containing a transcription stop signal and a y+t* marker separates a αTub84B promoter from a mutated hpo coding region that carries mutations in 4 of the 5 autophosphorylation sites of the linker region (T356A, T374A, T387A, T421A). The 5th autophosphorylation site T431 has not been mutated (this residue is also part of a mats protein binding site in the wild-type protein).

(Zheng et al., 2017)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{αTub(FRT.y+)FLAG-hpo.4A-431T}
(FlyBase, 1992-)
hpo4A/431T
(Zheng et al., 2017)
tub-FRT-y+-FRT- FLAG-hpo4A/431T
(Kaya-Çopur et al., 2021)
Secondary FlyBase IDs
    References (4)