The P{attP.loxP(y+)} element acts as a landing site for integration of DNA into the genome at a defined location; the element contains an attP site at one end, which acts as a target site for phiC31:int-mediated integration of any attB site-containing donor plasmid. P{attP.loxP(y+)} was generated in vivo from P{RLY}, using phiC31:Dint(KEE) disintegrase to regenerate an attP site from the attL and attR sites present in P{RLY}.