FB2026_02 , released June 18, 2026
Recombinant Construct: P{UAS(FRT.stop)mCD8::GFP-2A-mCherry::Syt1}
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General Information
Symbol
P{UAS(FRT.stop)mCD8::GFP-2A-mCherry::Syt1}
FlyBase ID
FBtp0131957
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPFRT.UAS.2A.Tag:M(mCd8a)
Syt1FRT.UAS.2A.mCherry
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Syt1FRT.UAS.2A.mCherry
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Syt1
Tagged with
mCherry
Tag:APEP
Also carries
FRT
Description

Twenty copies of UAS regulatory sequences are separated from Tag:M(mCd8a)-GFP followed by a "T2A" peptide from Thosea asigna, Tag:APEP (a consensus acceptor peptide for biotin ligase, with an additional glutamate residue at the C-terminal end), and the Syt1 coding sequence tagged with mCherry by a FRT flanked transcriptional stop cassette. The T2A peptide ensures that Tag:M(mCd8a)-GFP and mCherry ::Syt1 are produced as separate, individual proteins during translation.

(Urwyler et al., 2015)
Component allele Avic\GFPFRT.UAS.2A.Tag:M(mCd8a)
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
GFP
Tagged with
Tag:M(mCd8a)
Also carries
FRT
Description

Twenty copies of UAS regulatory sequences are separated from Tag:M(mCd8a)-GFP followed by a "T2A" peptide from Thosea asigna, Tag:APEP (a consensus acceptor peptide for biotin ligase, with an additional glutamate residue at the C-terminal end), and the Syt1 coding sequence tagged with mCherry by a FRT flanked transcriptional stop cassette. The T2A peptide ensures that Tag:M(mCd8a)-GFP and mCherry ::Syt1 are produced as separate, individual proteins during translation.

(Urwyler et al., 2015)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
20xUAS-FRT>STOP>FRT-mCD8::GFP-2A-mCherry::Syt1
(Urwyler et al., 2015)
P{UAS(FRT.stop)mCD8::GFP-2A-mCherry::Syt1}
(FlyBase, 1992-)
UAS-FRT>STOP>FRT-CD8::GFP-2A-APEP-mCherry::Syt1
(Urwyler et al., 2015)
Secondary FlyBase IDs
    References (3)