FB2026_02 , released June 18, 2026
Recombinant Construct: P{UAS-mito-QC}
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General Information
Symbol
P{UAS-mito-QC}
FlyBase ID
FBtp0131997
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
UAS-mito-QC, UAS-mitoQC, mito-QC
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Description

UASt regulatory sequences drive expression of the 'mito-QC' mitophagy reporter, which consists of the mCherry and GFP coding sequences in tandem and fused to the Tag:Mito(hFIS1) outer mitochondrial membrane localization signal. Under normal conditions, fluorescence from both mCherry and GFP occurs. Upon mitophagy and incorporation of the mitochondrion into a lysosome, the low pH of the lysosome quenches the GFP signal but not the mCherry signal (PMID:24176932).

(Lee et al., 2018)
Construct components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
GFP
mCherry
Tagged with
Tag:Mito(hFIS1)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (2)
Stocks containing insertion(s) of this recombinant construct
Bloomington
91641
w1118; P{UAS-mito-QC}attP2
91640
w1118; P{UAS-mito-QC}attP16
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (17)