UAS regulatory sequences drive expression of a truncated form of Hsap\ATXN3, consisting of a Q repeat region of 76 residues (which contains both α-helical coiled-coil and β-sheet hairpin structures) flanked upstream by 14 amino acids and downstream by 43 amino acids. The protein is tagged at the N-terminal end with Tag:HA and at the C-terminal end with Tag:MYC.
UAS regulatory sequences drive expression of a truncated form of Hsap\ATXN3, consisting of a Q repeat region of 76 residues (which contains both α-helical coiled-coil and β-sheet hairpin structures) flanked upstream by 14 amino acids and downstream by 43 amino acids. The protein is tagged at the N-terminal end with Tag:HA and at the C-terminal end with Tag:MYC.