UASt regulatory sequences drive expression of a full-length Mctp cDNA in which all the predicted calcium coordinating residues in the C2A, C2B and C2C domain have been mutated (amino acid substitutions D233N, D239N, D286N, D288N, D294N, D387N, D393N, D534N, D540N, D586N, D588N and E594N). The coding sequence is tagged with Tag:FLAG.