FB2026_02 , released June 18, 2026
Recombinant Construct: PBac{U6:3-Snap24-Snap25.gRNA}
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General Information
Symbol
PBac{U6:3-Snap24-Snap25.gRNA}
FlyBase ID
FBtp0134092
Feature type
transgenic_transposable_element
Component Allele(s)
Snap24U6:3.gRNA.Snap24-Snap25
Snap25U6:3.gRNA.Snap24-Snap25
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Snap24U6:3.gRNA.Snap24-Snap25
Transgenic product class
sgRNA
(Poe et al., 2019)
Component
Use(s)
Regulatory region(s)
snRNA:U6:96Ac
Encoded product / tool
Snap24
Description

A snRNA:U6:96Ac promoter drives expression of four sgRNAs; two (CCTCATGGACGAAAGCAAGG and ATTGATGAGCGGGAACGCGG) target Snap24 for knockout and two (CTCGGTCTTCGGGACCTGAG and GTAGCCCTTGATGATCAAGG) target Snap25 for knockout. The sgRNAs are expressed as a polycistronic cassette based on the tRNA-gRNA design of FBrf0233589.

(Poe et al., 2019)
Component allele Snap25U6:3.gRNA.Snap24-Snap25
Transgenic product class
sgRNA
(Poe et al., 2019)
Component
Use(s)
Regulatory region(s)
snRNA:U6:96Ac
Encoded product / tool
Snap25
Description

A snRNA:U6:96Ac promoter drives expression of four sgRNAs; two (CCTCATGGACGAAAGCAAGG and ATTGATGAGCGGGAACGCGG) target Snap24 for knockout and two (CTCGGTCTTCGGGACCTGAG and GTAGCCCTTGATGATCAAGG) target Snap25 for knockout. The sgRNAs are expressed as a polycistronic cassette based on the tRNA-gRNA design of FBrf0233589.

(Poe et al., 2019)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
81908
w*; PBac{U6:3-Snap24-Snap25.gRNA}VK00027
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
PBac{U6:3-Snap24-Snap25.gRNA}
(FlyBase, 1992-)
Snap24/25gRNA
(Ochi et al., 2024)
gRNA-Snap24-Snap25
(Poe et al., 2019)
Secondary FlyBase IDs
    References (4)