FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: PBac{U6:3-GFP.gRNA.ACMG(FE)}
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General Information
Symbol
PBac{U6:3-GFP.gRNA.ACMG(FE)}
FlyBase ID
FBtp0134101
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPU6:3.gRNA
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Description

A snRNA:U6:96Ac ( U6:3 ) promoter drives expression of a dual-gRNA in which a Drosophila tRNA-Gly (G) is followed by a gRNA targeting EGFP, and a second tRNA-Gly (G) is followed by a gRNA targeting GFP. The (F+E) gRNA scaffold described in FBrf0241409 is used.

(Koreman et al., 2021)
Construct components
Component allele Avic\GFPU6:3.gRNA
Transgenic product class
sgRNA
(Poe et al., 2019)
Component
Use(s)
Regulatory region(s)
snRNA:U6:96Ac
Encoded product / tool
Avic\GFP
Description

A snRNA:U6:96Ac promoter drives expression of two sgRNAs (CAACTACAAGACCCGCGCCG and GGTTGTCTGGTAAAAGGACA), each of which target GFP for knockout. The sgRNAs are expressed as a polycistronic cassette based on the tRNA-gRNA design of FBrf0233589.

(Poe et al., 2019)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
81897
w*; PBac{U6:3-GFP.gRNA.ACMG(FE)}VK00027
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
PBac{U6:3-GFP.gRNA.ACMG(FE)}
(FlyBase, 1996-)
PBac{U6:3-GFP.gRNA}
(FlyBase, 1996-)
gRNA-GFP
(Poe et al., 2019)
gRNA-GFPACMG(FE)
(Koreman et al., 2021)
Secondary FlyBase IDs
    References (4)