FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{spok-dCas9.H}
Open Close
General Information
Symbol
P{spok-dCas9.H}
FlyBase ID
FBtp0135599
Feature type
transgenic_transposable_element
Component Allele(s)
Spyo\Cas9dCas9.spok.cHa
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Spyo\Cas9dCas9.spok.cHa
Transgenic product class
Component
Use(s)
Regulatory region(s)
spok
Encoded product / tool
dCas9(D10A.N863A)
Tagged with
Tag:NLS(Unk)
Description

The spok regulatory region drives expression of dCas9(D10A.N863A). The coding sequence is tagged with nuclear localization signals at both the N-terminal and C-terminal ends of the open reading frame. The transgene can be used for transcription interference (CRISPRi) in combination with an sgRNA that targets dCas9(D10A.N863A) to a promoter; the promoter-bound dCas9(D10A.N863A) can sterically interfere with the formation of the pre-initiation complex, disrupting transcription.

(Huynh et al., 2018)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{spok-dCas9.H}
(FlyBase, 1992-)
Spok.dCas9
(Huynh et al., 2018)
Spok_dI
(Huynh et al., 2018)
Secondary FlyBase IDs
    References (3)