FB2026_02 , released June 18, 2026
Recombinant Construct: P{Tub-DBS-S-H2Av-GFP}
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General Information
Symbol
P{Tub-DBS-S-H2Av-GFP}
FlyBase ID
FBtp0137235
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Description

A tubulin promoter drives expression of a Drice-based sensor (DBS) of initiator caspase activity. The sensor is a fusion protein composed of Tag:M(mCd8a) transmembrane domains, a mutated form of Drice that carries a C211A mutation in the catalytic site (rendering it inactive) and that is truncated such that only 16 amino acid residues remain after the the initiator caspase cleavage site TETD/G, and a fluorescent His2Av-GFP moiety. In the absence of caspase activation the fusion protein is tethered to the cellular membranes outside the nucleus (due to the Tag:M(mCd8a) tag). In the presence of an initiator caspase, the Drice sequence can be cleaved at the TETD/G site, releasing the His2Av-GFP moiety and allowing its translocation to the nucleus.

Construct components
Component
Use(s)
Encoded product / tool
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (2)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{Tub-DBS-S-H2Av-GFP}
Secondary FlyBase IDs
    References (3)