FlyBase Recombinant Construct Report: P{UAS-mito.RFP.ER.G}

UASt regulatory sequences drive expression of mRFP(Unk) that is tagged at the N-terminal end with a Tag:Mito(mAkap1) mitochondrial localization signal and at the C-terminal end with a Tag:ER(yUBC6) endoplasmic reticulum localization sequence. This results in a protein that can be used to tighten the physical coupling between the ER and mitochondria, with the mRFP(Unk) protein acting as a linker (as well as being fluorescent); the maximum length of the protein, based on the size of mRFP(Unk) is calculated to be less than 5nm.

(Garrido-Maraver et al., 2020)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Disc\RFP^{mRFP.UAS.cGa.Tag:Mito(mAkap1),Tag:ER(yUBC6)}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

P{UAS-mAKAP1-mRFP-yUBC6}

(FlyBase, 1992-)

P{UAS-mito.RFP.ER.G}

(FlyBase, 1996-)

UAS-Linker

(Maddison et al., 2023)

UAS-linker

(Aboufares El Alaoui et al., 2023, Garrido-Maraver et al., 2020)

Secondary FlyBase IDs

References (6)

Report Sections

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