FB2026_02 , released June 18, 2026
Recombinant Construct: P{matTub-Cdk1-FRET}
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General Information
Symbol
P{matTub-Cdk1-FRET}
FlyBase ID
FBtp0141644
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPCdk1-FRET.matTub
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPCdk1-FRET.matTub
Transgenic product class
Component
Use(s)
Encoded product / tool
Avic\GFP
Description

A maternal Tubulin promoter drives expression of a fluorescence resonance energy transfer (FRET)-based biosensor for Cdk1 activity. The sqh 3'UTR is also present downstream of the coding sequence. The biosensor is composed of two fluorescent proteins (YPet and mCer) which are separated by sequence comprising a Cdk1 phosphorylation site from cyclin B1 and the polo box domain of Plk1. Phosphorylation of the Cdk1 phosphorylation site results in a conformational change that increases FRET efficiency.

(Deneke et al., 2016)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Cdk1-FRET
(Deneke et al., 2019)
Cdk1-FRET sensor
(Deneke et al., 2016)
P{matTub-Cdk1-FRET}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (4)