M{UAS-ovPrP.VRQ.cyt.RFP-} was generated in vivo by excision from M{UAS-ovPrP.VRQ.cyt} of the loxP cassette that contains Disc\RFP3xP3.PB.
UASt regulatory sequences drive expression of mature length Oari\PrP (amino acid residues 25-232) encoding the naturally occurring "VRQ" polymorphic variant (Val[136] Leu[141] Arg[154] Gln[171]). The encoded protein is expected to be cytosolic.