P{Act5C-AsCas12a.P.w-.RFP+} was generated in vivo from P{Act5C-AsCas12a.P}, using P{white-eraser} to exchange the miniwhite marker for a Disc\RFPDsRed.3xP3.cUa marker.
Act5C regulatory sequences drive expression of the AspBV3L6\Cas12a RNA-guided nuclease.