FB2026_02 , released June 18, 2026
Recombinant Construct: P{UAS-EGFP-Rab6-TAG-Nluc}
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General Information
Symbol
P{UAS-EGFP-Rab6-TAG-Nluc}
FlyBase ID
FBtp0143344
Feature type
transgenic_transposable_element
Component Allele(s)
Rab6UAS.EGFP.TAG.NanoLuc
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Rab6UAS.EGFP.TAG.NanoLuc
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Rab6
Tagged with
EGFP
NanoLuc
Description

UAS regulatory sequences are fused upstream of Rab6, including its native stop codon and extension 3'UTR. The Rab6 coding sequence is tagged at the N-terminal end with EGFP. In addition, the coding sequence for NanoLuc (missing a start codon) has been inserted immediately upstream of the second in-frame stop codon in the extension 3'UTR. If translation terminates at the native Rab6 stop codon, the NanoLuc reporter is not translated. If translational readthrough occurs past the stop codon, the Rab6 protein produced is tagged with the NanoLuc reporter.

(Chen et al., 2020)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{UAS-EGFP-Rab6-TAG-Nluc}
(FlyBase, 1992-)
UAS-eGFP-Rab6-TAG-Nluc
(Chen et al., 2020)
UAS-egfp-rab6-UTR-STOP-nluc
(Chen et al., 2020)
Secondary FlyBase IDs
    References (3)