tetO regulatory sequences are fused upstream of two minimal promoters (Hsp70 and P\T) followed by a reporter cassette that consists of the pLUC and EGFP open reading frames separated by a T2A motif (to ensure separate translation of each protein).
tetO regulatory sequences are fused upstream of two minimal promoters (Hsp70 and P\T) followed by a reporter cassette that consists of the pLUC and EGFP open reading frames separated by a T2A motif (to ensure separate translation of each protein).