FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: TI{20XUAS-lexA::p65-OR-FLP}
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General Information
Symbol
TI{20XUAS-lexA::p65-OR-FLP}
FlyBase ID
FBtp0144304
Feature type
engineered_region
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Description

TI{20XUAS-lexA::p65-OR-FLP} is designed to generate mosaic tissues composed of clones which express either lexA::p65 or FLP and has the following structure: 20xUAS regulatory sequences - attB site - lexA::p65 sequence in inverted orientation relative to UAS - attP site - STOP sequence - attP site - FLP sequence. In the absence of phiC31:int recombinase neither lexA::p65 nor FLP is expressed. In the presence of phiC31:int, recombination can occur between the attB site and one of the two attP sites in a stochastic manner, with no further recombination possible. If recombination occurs between the attB site and the attP site upstream of the STOP sequence, the lexA::p65 sequence is inverted, putting it in the correct orientation for expression under the control of the UAS sequences (FLP is still not expressed due to the STOP sequence). If recombination instead occurs between the attB site and the attP site downstream of the STOP sequence, lexA::p65 and the STOP sequence are excised, allowing expression of FLP. The first recombination event (resulting in lexA::p65 expression) occurs at a higher frequency than the second recombination event.

Construct components
Component
Use(s)
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
TI{20XUAS-Lex-OR-Flp}
TI{20XUAS-lexA::p65-OR-FLP}
Secondary FlyBase IDs
    References (2)