FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: PBac{βTub85D-Cas9.F}
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General Information
Symbol
PBac{βTub85D-Cas9.F}
FlyBase ID
FBtp0144821
Feature type
transgenic_transposable_element
Component Allele(s)
Spyo\Cas9βTub85D.PF
Size
Expression Data
Associated insertion(s)
6 available
Molecular map
Description and Uses
Construct components
Component allele Spyo\Cas9βTub85D.PF
Transgenic product class
Component
Use(s)
Regulatory region(s)
βTub85D
Encoded product / tool
Cas9
Tagged with
Tag:NLS(SV40-largeT)
Tag:NLS(Unk)
Description

A 478bp βTub85D 5' regulatory fragment drives expression of human codon-optimized Cas9 that is tagged with nuclear localization signals at both the N-terminal and C-terminal ends of the open reading frame. A 651bp βTub85D 3'UTR fragment is also present.

(Fasulo et al., 2020)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PBac{βTub85D-Cas9.F}
(FlyBase, 1996-)
βtub85D-cas9
(Fasulo et al., 2020)
Secondary FlyBase IDs
    References (3)