FlyBase Recombinant Construct Report: P{UAS-scFv235.HA}

UASt regulatory sequences drive expression of 'ScFv235', a single-chain variable fragment antibody that has previously been shown to bind selectively to the phospho-serine 396/404 epitope of Hsap\MAPT and to detect only the full-length phosphorylated protein and not degraded Hsap\MAPT fragments (PMID:25505335). The coding sequence is tagged at the N-terminal end with a signal peptide (so that it is secreted) and at the C-terminal end with Tag:polyHis and Tag:HA.

(Krishnaswamy et al., 2020)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Zzzz\scFv^{235.UAS.Tag:polyHis,Tag:HA}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

P{UAS-scFv235.HA}

(FlyBase, 1992-)

UAS-scFv235

(Krishnaswamy et al., 2020)

Secondary FlyBase IDs

References (3)

Report Sections

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