FlyBase Recombinant Construct Report: P{U6-PE-e[G111X]}

General Information

Symbol

P{U6-PE-e^G111X}

FlyBase ID

FBtp0146220

Feature type

transgenic_transposable_element

Component Allele(s)

e^U6.PE-G111X

Size

Expression Data

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component allele e^U6.PE-G111X

Transgenic product class

Component

Use(s)

Regulatory region(s)

snRNA:U6:96Ac

Encoded product / tool

Description

The snRNA:U6:96Ac promoter drives expression of a prime editing guide RNA (pegRNA) that is designed to introduce a premature stop codon (amino acid replacement G111term) into the e locus when used as part of a Prime Editing system.

(Bosch et al., 2021)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

e^U6.PE-G111X

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

90969

y¹ v¹; P{U6-PE-e^G111X}attP40/CyO

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

P{U6-PE-e^G111X}

(FlyBase, 1992-)

pCFD3-PE-ebony^G111X

(Bosch et al., 2021)

Secondary FlyBase IDs

References (3)

Report Sections

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