FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-Cas9.T2A.eGFP}
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General Information
Symbol
P{UAS-Cas9.T2A.eGFP}
FlyBase ID
FBtp0147160
Feature type
transgenic_transposable_element
Component Allele(s)
Spyo\Cas9UAS.T2A.EGFP
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Spyo\Cas9UAS.T2A.EGFP
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Cas9
Tagged with
EGFP
Description

UASt regulates expression of Spyo\Cas9 with a nuclear localization signal (NLS) at its N and C termini. An EGFP sequence is present downstream of Spyo\Cas9, separated by a T2A sequence - EGFP acts as a reporter for Spyo\Cas9 expression, while the presence of the self-cleaving peptide T2A sequence ensured that two independent proteins are made and that the EGFP tag does not hinder the activity of Spyo\Cas9.

(Trivedi et al., 2020)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
94298
βTub60DPin-1/CyO; P{UAS-Cas9.T2A.eGFP}3/TM2
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-Cas9.T2A.eGFP}
(FlyBase, 1992-)
UAS-Cas9-T2A-eGFP
(Trivedi et al., 2020)
Secondary FlyBase IDs
    References (4)