FB2026_03 , released September 17, 2026
Recombinant Construct: P{UAS-mCh-T2A-UGA-321-T2A-GFP}
Open Close
General Information
Symbol
P{UAS-mCh-T2A-UGA-321-T2A-GFP}
FlyBase ID
FBtp0147383
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

Transgenic construct used to test the ability of a kel fragment to mediate stop codon readthrough. UAS regulatory sequences drive expression of a reporter in which two fluorescent proteins (mCherry and GFP) are separated by a kel gene test fragment (that extends from 84 nucleotides 5' to 321 nucleotides 3' relative to the UGA kel ORF1 stop codon) which is flanked on each side by a T2A sequence. Readthrough efficiency can be calculated from the ratio of GFP produced by this transgenic construct compared to a matched control in which the UGA stop codon of the kel fragment has been mutated to an in-frame UGG codon (P{UAS-mCh-T2A-UGG-321-T2A-GFP}).

(Hudson et al., 2021)
Construct components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
GFP
mCherry
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{UAS-mCh-T2A-UGA-321-T2A-GFP}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)