FB2026_02 , released June 18, 2026
Recombinant Construct: PBac{gRNA-40D4.nls.mTagBFP2}
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General Information
Symbol
PBac{gRNA-40D4.nls.mTagBFP2}
FlyBase ID
FBtp0147566
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Description

A snRNA:U6:96Ac promoter drives expression of two sgRNAs targeting a conserved intergenic region in cytological band 40D4 (near the centromere of chromosome arm 2L).

(Allen et al., 2021)
Construct components
Component
Use(s)
Marker(s)
Equa\eqFP578mTagBFP2.Ubi-p63E.Tag:NLS(SV40-largeT)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
92742
w*; PBac{gRNA-40D4.nls.mTagBFP2}VK00037/CyO, P{Wee-P.ph0}BaccWee-P20
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
PBac{gRNA-40D4.BFP}
(FlyBase, 1992-)
PBac{gRNA-40D4.nls.mTagBFP2}
(FlyBase, 1996-)
gRNA-40D4(BFP)
(Allen et al., 2021)
gRNA-40D4-nlsBFP
(Shen et al., 2026)
Secondary FlyBase IDs
    References (4)