FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{20xUAS-BiPOLES}
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General Information
Symbol
P{20xUAS-BiPOLES}
FlyBase ID
FBtp0148652
Feature type
transgenic_transposable_element
Component Allele(s)
Cnoc\ChR1::Gthe\ACR2BiPOLES.20xUAS
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Cnoc\ChR1::Gthe\ACR2BiPOLES.20xUAS
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Chrimson
GtACR2
Tagged with
mCer3
Description

20xUAS regulatory sequences (from pJFRC7) drive expression of 'BiPOLES', an optogenetic tool for bidirectional dual-color control of neuron activity. The 'BiPOLES' coding sequence contains both the GtACR2 and Chrimson optogenetic tools, allowing blue-light induced silencing (via the GtACR2 moiety) and red-light induced activation (via the Chrimson moiety) of neuronal cells. The GtACR2 and Chrimson components are joined by a linker composed of a membrane trafficking signal from Kir2.1, a mCer3 fluorescent protein and the transmembrane β helix of the rat gastric H+/K+ ATPase, to maintain the correct membrane topology of both GtACR2 and Chrimson.

(Vierock et al., 2021)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{20xUAS-BiPOLES}
(FlyBase, 1992-)
UAS-BiPOLES
(Vierock et al., 2021)
Secondary FlyBase IDs
    References (3)