FB2026_03 , released September 17, 2026
Recombinant Construct: Mi{2A-d2GFP-NLS.1}
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General Information
Symbol
Mi{2A-d2GFP-NLS.1}
FlyBase ID
FBtp0149716
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Description

Mi{2A-d2GFP-NLS.1} represents a transgenic construct generated in vivo by using phiC31:int-mediated recombination to replace the attP cassette of a Mi{MIC} element insertion with an intron phase 1 'Trojan' sequence cassette that consists of a splice acceptor site followed by: the T2A peptide - a destabilized form of GFP tagged with a nuclear localization signal - an Hsp70 transcription termination signal.

(Matsuda et al., 2021)
Construct components
Component
Use(s)
Encoded product / tool
GFP
Tagged with
Tag:NLS(Unk)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Mi{2A-d2GFP-NLS.1}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)