FB2026_03 , released September 17, 2026
Recombinant Construct: P{UAS-ERK-KTR(EE)-T2A-His2Av-mCh}
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General Information
Symbol
P{UAS-ERK-KTR(EE)-T2A-His2Av-mCh}
FlyBase ID
FBtp0150002
Feature type
transgenic_transposable_element
Component Allele(s)
Mmus\Elk1UAS.nKTR(EE)
Size
Expression Data
Associated insertion(s)
3 available
Molecular map
Description and Uses
Construct components
Component allele Mmus\Elk1UAS.nKTR(EE)
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Mmus\Elk1
Description

UASt regulatory sequences drive expression of a transcript that encodes two open reading frames separated by a T2A linker (this results in separate translation of each protein). The first ORF encodes a phosphomimetic variant (EE) of the Clover-based 'ERK-KTR' kinase translocation reporter of MAPK activity. The phosphomimetic mutations result in the ERK-KTR[EE] protein being constitutively located in the cytoplasm, regardless of endogenous MAPK activity levels. The second ORF encodes His2Av tagged with mCherry.

(Yuen et al., 2022)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (2)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
ERK-nKTREE
(Yuen et al., 2022)
P{UAS-EKR-KTR(EE)-T2A-His2Av-mCh}
(FlyBase, 1996-)
P{UAS-ERK-KTR(EE)-T2A-His2Av-mCh}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (5)