FB2026_02 , released June 18, 2026
Recombinant Construct: P{UAS-Xbp1.HA.GFP}
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General Information
Symbol
P{UAS-Xbp1.HA.GFP}
FlyBase ID
FBtp0150157
Feature type
transgenic_transposable_element
Component Allele(s)
Xbp1UAS.Tag:HA,GFP
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Xbp1UAS.Tag:HA,GFP
Transgenic product class
wild_type
(Cairrão et al., 2022)
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Xbp1
Tagged with
GFP
Tag:HA
Description

UASt regulatory sequences are fused upstream of the unspliced form of the Xbp1 transcript. This is followed by a Tag:HA tag (in frame with the unspliced form) and then a GFP tag (out of frame with the unspliced form). Under non-stress conditions, the unspliced form of the Xbp1 protein is produced, which is tagged with Tag:HA. Under conditions of ER stress, Ire1-mediated splicing results in a frameshift that brings the GFP tag into frame (and puts the Tag:HA tag out of frame) with the spliced form of the Xbp1 protein.

(Cairrão et al., 2022)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
605357
w*; snaSco/CyO; P{UAS-Xbp1.HA.GFP}attP2
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-Xbp1.HA.GFP}
(FlyBase, 1992-)
pUAST-Xbp1-HA-GFP
(Cairrão et al., 2022)
Secondary FlyBase IDs
    References (4)