FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: PBac{αTub67C-EGFP-bcd-LEXY(-FRT)sqh3'UTR}
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General Information
Symbol
PBac{αTub67C-EGFP-bcd-LEXY(-FRT)sqh3'UTR}
FlyBase ID
FBtp0150359
Feature type
transgenic_transposable_element
Component Allele(s)
bcdαTub67C.EGFP,Tag:LEXY.sqh3'UTR
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele bcdαTub67C.EGFP,Tag:LEXY.sqh3'UTR
Transgenic product class
cDNA
(Singh et al., 2022)
wild_type
(Singh et al., 2022)
Component
Use(s)
Regulatory region(s)
αTub67C
Encoded product / tool
bcd
Tagged with
EGFP
Tag:LEXY
Also carries
FRT
Description

The FRT cassette present in bcdαTub67C.EGFP,Tag:LEXY.FRT.bcd3'UTR.sqh3'UTR has been excised, removing the bcd 3'UTR and Disc\RFP3xP3.cUa marker sequence. This leaves a transgene in which the αTub67C promoter is fused upstream of wild-type bcd coding sequence (from cDNA) that is tagged at the N-terminal end with EGFP and at the C-terminal end with Tag:LEXY. A single FRT site and the sqh 3'UTR are present downstream of the bcd coding sequence. The bcd protein is expressed uniformly along the anterior-posterior axis of the embryo.

(Singh et al., 2022)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
PBac{αTub67C-EGFP-bcd-LEXY(-FRT)sqh3'UTR}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (3)