FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-hATXN3.80Q-CAG.HA}
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General Information
Symbol
P{UAS-hATXN3.80Q-CAG.HA}
FlyBase ID
FBtp0150869
Feature type
transgenic_transposable_element
Component Allele(s)
Hsap\ATXN380Q-CAG.UAS.Tag:HA
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Hsap\ATXN380Q-CAG.UAS.Tag:HA
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsap\ATXN3
Tagged with
Tag:HA
Description

UAS regulatory sequences drive expression of full-length Hsap\ATXN3, which has been engineered to encode an expanded polyglutamine (polyQ) repeat of 80 residues (the polyQ repeats are encoded by pure CAG repeats). The polyQ-reading frame is tagged at the C-terminal end with Tag:HA, while the other two sense reading frame alternatives are tagged with in-frame Tag:MYC and Tag:V5 respectively. Only blotting for the Tag:HA epitope gives a clear protein signal above background, indicating that repeat-associated non-ATG (RAN) translation is unlikely to be occurring from this transgene in vivo.

(Johnson et al., 2022)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
ATXN3-Q80(CAG)
(Johnson et al., 2022)
P{UAS-hATXN3.80Q-CAG.HA}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (3)