FB2026_03 , released September 17, 2026
Recombinant Construct: P{UASp-Arf1.I46D}
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General Information
Symbol
P{UASp-Arf1.I46D}
FlyBase ID
FBtp0158301
Feature type
transgenic_transposable_element
Component Allele(s)
Arf1I46D.UAS
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Arf1I46D.UAS
Transgenic product class
missense_variant
(Rodrigues et al., 2016)
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Arf1
Description

UASp regulates expression of Arf1 cDNA (LD24904) with the amino acid change I46D that makes its GTPase activity highly insensitive to activation by ASAP1 and weakly insensitive to activation by AGAP1 or Arf GAP1. The shRNA region targeted by P{UASp-Arf1.shRNA2} is mutated (with the encoded protein sequence maintained) to make it resistant to RNAi by P{UASp-Arf1.shRNA2}.

(Rodrigues et al., 2016)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UASp-Arf1.I46D}
(FlyBase, 1992-)
arf1I46D
(Rodrigues et al., 2016)
Secondary FlyBase IDs
    References (3)