FB2026_02 , released June 18, 2026
Recombinant Construct: M{UAS-N-Rok::2m22}
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General Information
Symbol
M{UAS-N-Rok::2m22}
FlyBase ID
FBtp0158738
Feature type
transgenic_transposable_element
Component Allele(s)
RokN.UAS.DARPin(2m22)
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele RokN.UAS.DARPin(2m22)
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rok
Tagged with
DARPin(2m22)
Description

UASt regulatory sequences drive expression of an engineered kinase that is composed of the N-terminal kinase domain of Rok fused to the DARPin(2m22) nanobody. The lack of the C-terminal domain of Rok results in the kinase domain being constitutively active, and the presence of the DARPin(2m22) nanobody should target this active kinase domain to proteins that are tagged with mCherry (the target of the DARPin(2m22) nanobody).

(Lepeta et al., 2022)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
M{UAS-N-Rok::2m22}
(FlyBase, 1992-)
UAS_N-Rok::2m22
(Lepeta et al., 2022)
Secondary FlyBase IDs
    References (3)