FlyBase Recombinant Construct Report: P{Flag.GFP.dTSSK}

The Tssk endogenous promoter and the genomic region covering the Tssk gene locus (from ATG to 1 kb downstream of the coding region amplified from w¹¹¹⁸ genomic DNA), tagged with GFP and Tag:FLAG, cloned into the pUAST-GFP-attB vector.

(Zhang et al., 2023)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Tssk^GFP,Tag:FLAG

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

testis | restricted

(Zhang et al., 2023)

elongation stage spermatid

(Zhang et al., 2023)

mid-late elongation-stage spermatid

(Zhang et al., 2023)

spermatid

(Zhang et al., 2023)

Additional Information

Statement

Reference

Expression is observed in whole testes in postmeiotic spermatid bundles, with bright spots scattered over the bundles. The bright spots are mainly located on the nucleus, flagellum, and individualization complex of spermatids. They are mainly observed from the round stage to the canoe stage.

(Zhang et al., 2023)

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

P{Flag-GFP-dTSSK}

(Zhang et al., 2023)

P{Flag.GFP.dTSSK}

(FlyBase, 1992-)

Secondary FlyBase IDs

References (3)

Report Sections

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