FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: M{UAS-dCas9-Rbf}
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General Information
Symbol
M{UAS-dCas9-Rbf}
FlyBase ID
FBtp0161417
Feature type
transgenic_transposable_element
Component Allele(s)
RbfUAS.dCas9,Tag:FLAG
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele RbfUAS.dCas9,Tag:FLAG
Transgenic product class
wild_type
(Raicu et al., 2024)
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rbf
Tagged with
dCas9(D10A.H840A)
Tag:FLAG
Description

UASt sequences regulate expression of Rbf, tagged at the N-terminal end with both 2xTag:FLAG and dCas9(D10A.H840A) and at the C-terminal end with 2xTag:FLAG. The presence of the dCas9(D10A.H840A) sequence means that the Rbf protein can be recruited to specific promoters using appropriate gene-specific guide RNAs.

(Raicu et al., 2024)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (2)
Stocks containing insertion(s) of this recombinant construct
Bloomington
99958
w1118; M{UAS-dCas9-Rbf}ZH-86Fb
602359
w1118; P{GawB}nub-AC-62; M{UAS-dCas9-Rbf}ZH-86Fb
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
M{UAS-dCas9-Rbf}
(FlyBase, 1996-)
UAS:dCas9-Rbf1
(Raicu et al., 2024)
Secondary FlyBase IDs
    References (4)