FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: M{UAS-dCas9-Rbf.ΔIE}
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General Information
Symbol
M{UAS-dCas9-Rbf.ΔIE}
FlyBase ID
FBtp0161420
Feature type
transgenic_transposable_element
Component Allele(s)
RbfΔIE.UAS.dCas9,Tag:FLAG
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele RbfΔIE.UAS.dCas9,Tag:FLAG
Transgenic product class
inframe_deletion
(Raicu et al., 2024)
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rbf
Tagged with
dCas9(D10A.H840A)
Tag:FLAG
Description

UASt sequences regulate expression of a mutated form of Rbf that lacks the IE domain (residues 728-786 are deleted). The coding sequence is tagged at the N-terminal end with both 2xTag:FLAG and dCas9(D10A.H840A) and at the C-terminal end with 2xTag:FLAG. The presence of the dCas9(D10A.H840A) sequence means that the Rbf protein can be recruited to specific promoters using appropriate gene-specific guide RNAs.

(Raicu et al., 2024)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
99961
w1118; M{UAS-dCas9-Rbf.ΔIE}ZH-86Fb
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
M{UAS-dCas9-Rbf.ΔIE}
(FlyBase, 1996-)
UAS:dCas9-Rbf1ΔIE
(Raicu et al., 2024)
Secondary FlyBase IDs
    References (4)