TI{Trojan-dVP16AD.0} represents a transgenic construct generated in vivo by using phiC31:int-mediated recombination to replace the attP-flanked recombination-mediated cassette exchange (RMCE) cassette of a 'CRIMIC' element insertion with an intron phase 0 'Trojan dVP16AD' cassette from the pBS-KS-attB2-SA(0)-T2A-dVP16AD-Hsp70 plasmid. The Trojan dVP16AD cassette consists of a splice acceptor site followed by the T2A peptide, sequence encoding a VP16(AD)::Zip+ hemidriver and an Hsp70 transcription termination signal. Integration of the cassette into a CRIMIC insertion in a coding intron (with the same phase) of a native Drosophila gene of interest will result in the cassette behaving as a 'Trojan' exon: the splice acceptor site ensures that the T2A-VP16(AD)::Zip+ open reading frame is incorporated into the mRNA of the native Drosophila gene, while the T2A sequence truncates the native gene product and promotes the separate translation of the VP16(AD)::Zip+ open reading frame. Thus the VP16(AD)::Zip+ hemidriver should be expressed under the control of the regulatory sequences of the native Drosophila gene of interest in the resulting fly line.