Transgenic construct that expresses four sgRNAs that target three genes. Consists of the snRNA:U6:96Ac promoter driving expression of a sgRNA that targets eve, a snRNA:U6:96Aa driving expression of a sgRNA that targets hid, a gypsy insulator, a snRNA:U6:96Ac promoter driving expression of a sgRNA that targets wg, a snRNA:U6:96Aa driving expression of a second sgRNA that targets wg.