CRISPR construct designed to result in an indel (small insertion or deletion mutation) in a target gene when combined with a source of Cas9 and GAL4. UAS regulatory sequences drive expression of two sgRNAs that each target the coding sequence of a gene of interest: sgRNA1 targets Fas1, sgRNA2 targets mfas.
CRISPR construct designed to result in an indel (small insertion or deletion mutation) in a target gene when combined with a source of Cas9 and GAL4. UAS regulatory sequences drive expression of two sgRNAs that each target the coding sequence of a gene of interest: sgRNA1 targets Fas1, sgRNA2 targets mfas.