FB2026_03 , released September 17, 2026
Recombinant Construct: P{nanos-Target-AID}
Open Close
General Information
Symbol
P{nanos-Target-AID}
FlyBase ID
FBtp0168339
Feature type
transgenic_transposable_element
Component Allele(s)
Spyo\Cas9Target-AID.nanos
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Spyo\Cas9Target-AID.nanos
Transgenic product class
Component
Use(s)
Regulatory region(s)
nanos
Encoded product / tool
Spyo\Cas9
Description

The nanos promoter drives expression of 'Target-AID', a base editor that targets cytosine in DNA; it favors the conversion of C to T. The 'Target-AID' sequence (from Addgene plasmid 79620) encodes a fusion protein composed of 1. an engineered Cas9 nickase (contains the D10A mutation), 2. cytidine deaminase 1 from sea lamprey (PmCDA1) and 3. the uracil DNA glycosylase inhibitor (UGI) from a Bacillus subtilis bacteriophage (see PMID:27492474).

(Thakkar et al., 2023)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{nanos-Target-AID}
(FlyBase, 1992-)
nos-Target-AID
(Thakkar et al., 2023)
Secondary FlyBase IDs
    References (3)