FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{Tub(-FRT)yki.P88L.3SA.EGFP}
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General Information
Symbol
P{Tub(-FRT)yki.P88L.3SA.EGFP}
FlyBase ID
FBtp0169842
Feature type
transgenic_transposable_element
Component Allele(s)
ykiP88L.3SA.αTub84B.EGFP
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele ykiP88L.3SA.αTub84B.EGFP
Transgenic product class
missense_variant
(Parker and Struhl, 2015)
polypeptide_post_translational_processing_variant
(Parker and Struhl, 2015)
Component
Use(s)
Regulatory region(s)
αTub84B
Encoded product / tool
yki
Tagged with
EGFP
Also carries
FRT
Description

The FRT cassette has been excised, allowing the αTub84B promoter to drive expression of a full-length yki cDNA that has been mutated to carry a P88L amino acid substitution (blocks nuclear accumulation in response to mTor inhibition) plus three substitutions of phosphorylation sites (S111A, S168A and S250A). In addition, the coding sequence is tagged at the C-terminal end with EGFP.

(Parker and Struhl, 2015)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{Tub(-FRT)yki.P88L.3SA.EGFP}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (3)