FB2026_03 , released September 17, 2026
Recombinant Construct: P{UAS-dCas9::KRAB}
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General Information
Symbol
P{UAS-dCas9::KRAB}
FlyBase ID
FBtp0170962
Feature type
transgenic_transposable_element
Component Allele(s)
Spyo\Cas9dCas9::KRAB.UAS
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Spyo\Cas9dCas9::KRAB.UAS
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Spyo\Cas9
Description

UASt sequences regulate expression of 'dCas9' (a catalytically dead form of the Cas9 endonuclease) fused to the KRAB transcriptional repression domain from the Homo sapiens ZNF10 gene (HGNC:12879). The transgene can be used for transcription interference (CRISPRi) in combination with an sgRNA that targets the dCas9::KRAB fusion protein to the promoter region of a gene of interest.

(Schneider et al., 2023)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-dCas9::KRAB}
(FlyBase, 1992-)
UAS-dcas9:KRAB
(Schneider et al., 2023)
Secondary FlyBase IDs
    References (3)