FlyBase Recombinant Construct Report: TI{20XUAS-SPARC2-D-GtACR1::EYFP}

A "SPARC2-D" module separates 20XUAS regulatory sequences from sequence encoding a Drosophila codon-optimized version of GtACR1 that is tagged with EYFP. The SPARC2-D module comprises: 1. a STOP cassette that is flanked by two full-length (60bp) attP sites, 2. ribozyme sequence (to prevent leaky expression in the absence of phiC31:int) and 3. an attB site. phiC31:int catalyzes irreversible recombination between the attB site and one of the two attP sites; if the attP site upstream of the STOP cassette is utilized, the STOP cassette is excised, permitting expression of EYFP-tagged GtACR1, while if the attP site downstream of the STOP cassette is utilized, the STOP cassette is preserved.

(Yang et al., 2024)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Gthe\ACR1^{20XUAS.SPARC2-D.EYFP}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

TI{20XUAS-SPARC2-D-GtACR1::EYFP}

(FlyBase, 1996-)

UAS-SPARC2-D-GtACR1::eYFP

(Yang et al., 2024)

Secondary FlyBase IDs

References (3)

Report Sections

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