FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: PBac{20XUAS-IVS-jGCaMP8m-T2A-mCyRFP3}
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General Information
Symbol
PBac{20XUAS-IVS-jGCaMP8m-T2A-mCyRFP3}
FlyBase ID
FBtp0172522
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Description

20xUAS sequences regulate expression of a bicistronic transcript that encodes two open reading frames separated by a T2A self-cleaving peptide (to ensure separate translation of each protein). The first ORF encodes jGCaMP8m. The second ORF encodes mCyRFP3. The coding sequences are codon optimized for Drosophila.

(Wilson and Holtz, 2025.2.27)
Construct components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
jGCaMP8m
mCyRFP3
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
606332
w1118; PBac{20XUAS-IVS-jGCaMP8m-T2A-mCyRFP3}VK00005/TM3, Sb1
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
PBac{20XUAS-IVS-jGCaMP8m-T2A-mCyRFP3}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)