FB2026_03 , released September 17, 2026
Recombinant Construct: Mi{24xMS2-V6}
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General Information
Symbol
Mi{24xMS2-V6}
FlyBase ID
FBtp0172965
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Description

Mi{24xMS2-V6} represents a transgenic construct generated in vivo by using phiC31:int-mediated recombination to replace the attP cassette of a Mi{MIC} element insertion with a '24xMS2-V6' stem-loop cassette that contains 24 copies of Tag:MS2 (the cassette contains 2 tandem copies of a sequence encoding 12 unique Tag:MS2 stem loops). Each copy of Tag:MS2 contains a U instead of C at a crucial nucleotide in the Tag:MCP binding site that weakens the interaction and makes the Tag:MS2-Tag:MCP interaction less stable. Insertion of the Mi{24xMS2-V6} cassette in the correct orientation into an intron of a gene of interest allows visualization of nascent transcription of that gene; the Tag:MS2 sequences in the nascent transcript form stem loops that can be detected by a reporter protein that includes Tag:MCP (which binds Tag:MS2).

(Beadle et al., 2024)
Construct components
Component
Use(s)
Tagged with
Tag:MS2
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Mi{24xMS2-V6}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)