10xUAS regulatory sequences drive expression of the 2N4R isoform of Hsap\MAPT carrying 14 mutations to glutamates (Thr111, Thr153, Thr175, Thr181, Ser199, Ser202, Thr205, Thr212, Thr217, Thr231, Ser235, Ser396, Ser404 and Ser422); tagged with mCherry.