FB2026_03 , released September 17, 2026
Recombinant Construct: P{U6:3-Tie.6xgRNA}
Open Close
General Information
Symbol
P{U6:3-Tie.6xgRNA}
FlyBase ID
FBtp0175259
Feature type
transgenic_transposable_element
Component Allele(s)
TieU6:3.6xgRNA
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele TieU6:3.6xgRNA
Transgenic product class
sgRNA
(Choi et al., 2025)
Component
Use(s)
Regulatory region(s)
snRNA:U6:96Ac
Encoded product / tool
Tie
Description

The snRNA:U6:96Ac promoter drives expression of six gRNAs, consisting of two duplicated sets targeting three selected sites within the first and second common exons of Tie. The gRNA sequences are TTGAATGCGACCGCCGGCGA, CCCACTACTTATAGGCCCTC and CTGCCACCCATACGACCGGG.

(Choi et al., 2025)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{U6:3-Tie.6xgRNA}
(FlyBase, 1992-)
P{pCFD5w-6xgRNA-tie}
(Choi et al., 2025)
Secondary FlyBase IDs
    References (3)