FB2026_03 , released September 17, 2026
Recombinant Construct: P{Cas9.D10A.G4HACK}
Open Close
General Information
Symbol
P{Cas9.D10A.G4HACK}
FlyBase ID
FBtp0175537
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Description

Donor transgenic construct for use in the homology assisted CRISPR knock-in (HACK) method. Designed to convert a GAL4 line to a Cas9(D10A) line in vivo. The construct carries a 2A-Cas9(D10A) coding sequence flanked by GAL4 homology arms, a dual-gRNA cassette that ubiquitously expresses 2 gRNAs targeting GAL4 coding sequences (one using the 'CR7T' pol III promoter and the other using the snRNA:U6:96Ac promoter) and a ubiquitous nuclear mTagBFP2 marker. When combined with a source of Cas9 and the GAL4 line of interest, the gRNAs generate double-strand breaks in the middle of the GAL4 coding sequence and allow in-frame incorporation of 2A-Cas9(D10A) via homology-directed repair. The 'self-cleaving' 2A peptide will release Cas9(D10A) and a truncated and nonfunctional GAL4 as two separate proteins after translation.

(Shen et al., 2026)
Construct components
Component
Use(s)
Encoded product / tool
Cas9(D10A)
Marker(s)
Equa\eqFP578mTagBFP2.Ubi-p63E.Tag:NLS(SV40-largeT)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
607576
w*; P{Cas9.D10A.G4HACK}attP40/CyO, P{Wee-P.ph0}2
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
HACK(Gal4)-DONR(T2A-D10A)
(Shen et al., 2026)
P{Cas9.D10A.G4HACK}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)