FB2026_03 , released September 17, 2026
Recombinant Construct: M{Sce.white}
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General Information
Symbol
M{Sce.white}
FlyBase ID
FBtp0175586
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Description

The M{Sce.white} construct contains 'Sce.white', a copy of the w gene that is non-functional due to the insertion of an 18bp RS(I-SceI) site (plus an additional nucleotide) at the wild-type SacI site, resulting in an in-frame premature stop codon. A y+t* marker is also present downstream of the 'Sce.white' sequence. The construct acts as a target for repair in an assay to measure interhomolog homologous recombination (HR). Expression of I-SceI can induce a DSB at the RS(I-SceI) site in M{Sce.white}, and gene conversion of the 'Sce.white' sequence using a M{iwhite} or M{iwhiteΔ9} donor template at the same allelic locus on the homologous chromosome can produce a functional w gene, resulting in progeny with red or orange eyes respectively.

(Fernandez et al., 2019)
Construct components
Component
Use(s)
Also carries
RS(I-SceI)
Marker(s)
y+t*
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
M{Sce.white}
(FlyBase, 1996-)
Sce.white
(Fernandez et al., 2019)
Secondary FlyBase IDs
    References (2)