FB2026_03 , released September 17, 2026
Recombinant Construct: P{UAS-Lyso-GFP11x7-V5-P2A-FLAG-GFP(1-10)-ER}
Open Close
General Information
Symbol
P{UAS-Lyso-GFP11x7-V5-P2A-FLAG-GFP(1-10)-ER}
FlyBase ID
FBtp0175655
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPspGFP1-10.Lyso.UAS.Tag:FLAG,Tag:ER(cb5)
CG7523UAS.spGFP11.Tag:V5
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele CG7523UAS.spGFP11.Tag:V5
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
CG7523
Tagged with
spGFP11
Tag:V5
Description

UASt sequences regulate expression of a split-GFP sensor that can be used to quantify lysosome and endoplasmic reticulum (ER) contact. The construct contains a dicistronic transcription unit that encodes two open reading frames (ORFs) separated by a 2A linker (this promotes separate translation of each protein). The first ORF comprises full-length CG7523 (this targets the protein to the lysosomal membrane) fused to seven tandem copies of the spGFP11 split fluorescent protein fragment, followed by Tag:V5. The second ORF comprises Tag:FLAG fused to spGFP1-10 followed by a Tag:ER(cb5) ER targeting motif.

(Wang et al., 2026)
Component allele Avic\GFPspGFP1-10.Lyso.UAS.Tag:FLAG,Tag:ER(cb5)
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
spGFP1-10
Tagged with
Tag:ER(cb5)
Tag:FLAG
Description

UASt sequences regulate expression of a split-GFP sensor that can be used to quantify lysosome and endoplasmic reticulum (ER) contact. The construct contains a dicistronic transcription unit that encodes two open reading frames (ORFs) separated by a 2A linker (this promotes separate translation of each protein). The first ORF comprises full-length CG7523 (this targets the protein to the lysosomal membrane) fused to seven tandem copies of the spGFP11 split fluorescent protein fragment, followed by Tag:V5. The second ORF comprises Tag:FLAG fused to spGFP1-10 followed by a Tag:ER(cb5) ER targeting motif.

(Wang et al., 2026)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{UAS-Lyso-GFP11x7-V5-P2A-FLAG-GFP(1-10)-ER}
(FlyBase, 1992-)
UAS-Lyso-GFP11x7-V5-P2A-FLAG-GFP(1-10)-ER
(Wang et al., 2026)
UAS::Lyso-G11-G(1-10)-ER
(Wang et al., 2026)
Secondary FlyBase IDs
    References (3)